期刊论文详细信息
Frontiers in Nutrition
Comparative Analysis of in vitro Digestibility and Immunogenicity of Gliadin Proteins From Durum and Einkorn Wheat
Laura Gazza1  Renata Auricchio2  Serena Vitale3  Carmen Gianfrani3  Stefania Picascia3  Gianluca Picariello4  Luigia Di Stasio4  Gianfranco Mamone4 
[1] CREA—Research Centre for Engineering and Agro-Food Processing, Rome, Italy;Department of Translational Medical Science, Section of Paediatrics, European Laboratory for the Investigation of Food-Induced Diseases, University “Federico II”, Naples, Italy;Institute of Biochemistry and Cell Biology, National Research Council, Naples, Italy;Institute of Food Sciences, National Research Council, Avellino, Italy;
关键词: brush border membrane;    in vitro enzymatic digestion;    enzyme-linked immunosorbent assay (ELISA);    gluten proteins;    T-cell assay;    Triticum durum;   
DOI  :  10.3389/fnut.2020.00056
来源: DOAJ
【 摘 要 】

Recent studies suggested that gliadin proteins from the ancient diploid einkorn wheat Triticum monococcum retained a reduced number of immunogenic peptides for celiac disease patients because of a high in vitro digestibility with respect to hexaploid common wheat. In this study, we compared the immunological properties of gliadins from two Triticum monococcum cultivars (Hammurabi and Norberto-ID331) with those of a Triticum durum cultivar (Adamello). Gliadins were digested by mimicking the in vitro gastrointestinal digestion process that includes the brush border membrane peptidases. Competitive ELISA, based on R5 monoclonal antibody, showed that gastrointestinal digestion reduced the immunogenicity of Triticum monococcum gliadins; conversely, the immunogenic potential of Triticum durum gliadins remained almost unchanged by the in vitro digestion. The immune stimulatory activity was also evaluated by detecting the IFN-γ production in gliadin-reactive T-cell lines obtained from the small intestinal mucosa of HLA-DQ2+ celiac disease patients. Interestingly, gastrointestinal digestion markedly reduced the capability of Triticum monococcum gliadins (p <0.05) of both cultivars to activate T cells, while it slightly affected the activity of Triticum durum. In conclusion, our results showed that Triticum durum was almost unaffected by the in vitro gastrointestinal digestion, while Triticum monococcum had a marked sensibility to digestion, thus determining a lower toxicity for celiac disease patients.

【 授权许可】

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