CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities

Frontiers in Genetics

Wen Luo¹  Teng Xiong¹  Bhaskar Roy¹  Xiaodong Fang¹  Chao Yang¹  Yong Li¹  Yong Zhou¹  Lise Madsen²  Chabungbam O. Singh³  Jing Zhao⁴  Karsten Kristiansen⁴  Guanjun Gao⁵ 

[1] BGI Genomics, BGI-Shenzhen, Shenzhen, China;Institute of Marine Research, Bergen, Norway;Institute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou, China;Laboratory of Genomics and Molecular Biomedicine, Department of Biology, University of Copenhagen, Copenhagen, Denmark;School of Life Sciences, Tsinghua University, Beijing, China;

关键词: CRISPR;    Cascade 9;    genome editing;    off-target effects;    gene targeting;    RNA guided system;   

DOI  :  10.3389/fgene.2018.00240

来源: DOAJ

【 摘 要 】

Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and Cascade 9 (also known as Cas9, CRISPR associated protein 9) confer protection against invading viruses or plasmids. The CRISPR/Cascade 9 system constitutes one of the most powerful genome technologies available to researchers today. So far, this technology has enabled efficient genome editing and modification in several model organisms and has successfully been used in biomedicine and biomedical engineering. However, challenges for efficient and safe genetic manipulation in several organisms persist. Here, we review functional approaches and future challenges associated with the use of the CRISPR/Cascade 9 genome editing system and discuss opportunities, ethical issues and future directions within this field.

【 授权许可】

Unknown