Draft genomes of Cronobacter sakazakii strains isolated from dried spices bring unique insights into the diversity of plant-associated strains

Standards in Genomic Sciences

Kunho Seo¹  Ben D. Tall²  Youyoung Lee²  Samantha Finkelstein²  Hannah R. Chase²  Jungha Woo²  Junia Jean Gilles Beaubrun²  Nicole Addy²  Jayanthi Gangiredla²  Hyein Jang²  Isha Patel²  Flavia Negrete²  Laura Ewing²  Gopal R. Gopinath²  Ziad W. Jaradat³  Angelika Lehner⁴  Roger Stephan⁴  Athmanya Eshwar⁴  Srikumar Shabarinath⁵  Séamus Fanning⁵ 

[1] Center for One Health, College of Veterinary Medicine, Konkuk University;Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration;Department of Nutrition and Food Technology, Jordan University of Science and Technology;Institute for Food Safety and Hygiene, University of Zurich;UCD Centre for Food Safety, School of Public Health, Physiotherapy & Population Science, University College;

关键词: Cronobacter sakazakii;    WGS;    Draft Genomes;    Plant-origin;    Dried Spices;   

DOI  :  10.1186/s40793-018-0339-6

来源: DOAJ

【 摘 要 】

Abstract Cronobacter sakazakii is a Gram-negative opportunistic pathogen that causes life- threatening infantile infections, such as meningitis, septicemia, and necrotizing enterocolitis, as well as pneumonia, septicemia, and urinary tract and wound infections in adults. Here, we report 26 draft genome sequences of C. sakazakii, which were obtained from dried spices from the USA, the Middle East, China, and the Republic of Korea. The average genome size of the C. sakazakii genomes was 4393 kb, with an average of 4055 protein coding genes, and an average genome G + C content of 56.9%. The genomes contained genes related to carbohydrate transport and metabolism, amino acid transport and metabolism, and cell wall/membrane biogenesis. In addition, we identified genes encoding proteins involved in osmotic responses such as DnaJ, Aquaproin Z, ProQ, and TreF, as well as virulence-related and heat shock-related proteins. Interestingly, a metabolic island comprised of a variably-sized xylose utilization operon was found within the spice-associated C. sakazakii genomes, which supports the hypothesis that plants may serve as transmission vectors or alternative hosts for Cronobacter species. The presence of the genes identified in this study can support the remarkable phenotypic traits of C. sakazakii such as the organism’s capabilities of adaptation and survival in response to adverse growth environmental conditions (e.g. osmotic and desiccative stresses). Accordingly, the genome analyses provided insights into many aspects of physiology and evolutionary history of this important foodborne pathogen.

【 授权许可】

Unknown