Paenibacillus polymyxa A26 and Its Surfactant-Deficient Mutant Degradation of Polycyclic Aromatic Hydrocarbons

Stresses

Tiiu Teder¹  Salme Timmusk²  Lawrence Behers³ 

[1] Bashan Institute of Science, 1730 Post Oak Ct., Auburn, AL 36830, USA;Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, P.O. Box 7026, SE-75007 Uppsala, Sweden;Novawest Communications and Technologies, Tucson, AZ 85715, USA;

关键词: polycyclic aromatic hydrocarbons;    biodegradation;    Paenibacillus polymyxa A26;    Sfp-type 4-phosphopantetheinyl transferase;    exopolysaccharides;   

DOI  :  10.3390/stresses1040019

来源: DOAJ

【 摘 要 】

We compared the ability of two bacterial strains, Paenibacillus polymyxa A26 and P. polymyxa A26Sfp, for biodegradation of naphthalene (NAP). The studies were performed under simulated laboratory conditions, in liquid medium and soil with different carbon sources, pH and salt contents. Changes in the luminescence inhibition of Aliivibrio fischeri, as an indicator of the baseline toxicity, were observed in degradation mixtures during 7 days of incubation. While both strains expressed the best growth and NAP degradation ability in the minimal salt medium containing sucrose and 5% NaCl at pH 7 and 8, the mutant strain remained effective even under extreme conditions. A26Sfp was found to be an efficient and potentially industrially important polycyclic aromatic hydrocarbon degradation strain. Its extracellular polysaccharide production is 30%, and glucan production is twice that of the wild type A 26. The surface tension reduction ability was ascertained as 25–30% increased emulsification ability.

【 授权许可】

Unknown