Monitoring in vivo behavior of size-dependent fluorescent particles as a model fine dust

Journal of Nanobiotechnology

Young-Jun Park¹  Byeongwoo Goh²  Hye Sun Park²  Taewoong Son²  Youn-Joo Cho²  Sun-Hee Cho²  Kwan Soo Hong²  Mi Young Cho²  Hyun Min Kim²  Hyunseung Lee²  Phan Thi Ngoc Hoa² 

[1] Environmental Disease Research Center, Korea Research Institute of Bioscience and Biotechnology;Research Center for Bioconvergence Analysis, Korea Basic Science Institute;

关键词: Air pollution;    Particulate matter;    Silica particle;    Biodistribution;    In vivo image-tracking;   

DOI  :  10.1186/s12951-022-01419-4

来源: DOAJ

【 摘 要 】

Abstract Background There has been growing concern regarding the impact of air pollution, especially fine dust, on human health. However, it is difficult to estimate the toxicity of fine dust on the human body because of its diverse effects depending on the composition and environmental factors. Results In this study, we focused on the difference in the biodistribution of fine dust according to the size distribution of particulate matter after inhalation into the body to predict its impact on human health. We synthesized Cy7-doped silica particulate matters (CSPMs) having different particle sizes and employed them as model fine dust, and studied their whole-body in vivo biodistribution in BALB/c nude mice. Image-tracking and quantitative and qualitative analyses were performed on the ex vivo organs and tissues. Additionally, flow cytometric analysis of single cells isolated from the lungs was performed. Smaller particles with a diameter of less than 100 nm (CSPM0.1) were observed to be removed relatively rapidly from the lungs upon initial inhalation. However, they were confirmed to accumulate continuously over 4 weeks of observation. In particular, smaller particles were found to spread rapidly to other organs during the early stages of inhalation. Conclusions The results show in vivo behavioral differences that arisen from particle size through mouse experimental model. Although these are far from the human inhalation studies, it provides information that can help predict the effect of fine dust on human health. This study might provide with insights on association between CSPM0.1 accumulation in several organs including the lungs and adverse effect to underlying diseases in the organs. Graphical Abstract

【 授权许可】

Unknown