Discoidin Domain Receptor 1 Expression in Colon Cancer: Roles and Prognosis Impact

Cancers

Kaouther Ben Arfi¹  Camille Boulagnon-Rombi¹  Kevin Toussaint²  Nicole Bouland²  Christophe Schneider²  Amar Bennasroune²  Cuong Cao Le²  Cathy Hachet²  Aline Appert-Collin²  Stéphane Dedieu²  Guillaume Collin³  Olivier Bouché³  Véronique Lehrter³  Hamid Morjani³  Aurore Wolak-Thierry⁴ 

[1] Laboratoire de Biopathologie, Centre Hospitalier Universitaire de Reims, 51090 Reims, France;UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire (MEDyC), Université de Reims Champagne Ardenne (URCA), 51097 Reims, France;Unité BioSpecT, EA7506, Université de Reims Champagne Ardenne (URCA), 51096 Reims, France;Unité d’Aide Méthodologique, Centre Hospitalier Universitaire, 51100 Reims, France;

关键词: colon cancer;    discoidin domain receptor;    prognosis;    event free survival;    survival;   

DOI  :  10.3390/cancers14040928

来源: DOAJ

【 摘 要 】

Extracellular matrix components such as collagens are deposited within the tumor microenvironment at primary and metastatic sites and are recognized to be critical during tumor progression and metastasis development. This study aimed to evaluate the clinical and prognostic impact of Discoidin Domain Receptor 1 (DDR1) expression in colon cancers and its association with a particular molecular and/or morphological profile and to evaluate its potential role as a prognosis biomarker. Immunohistochemical expression of DDR1 was evaluated on 292 colonic adenocarcinomas. DDR1 was highly expressed in 240 (82.2%) adenocarcinomas. High DDR1 immunostaining score was significantly associated, on univariate analysis, with male sex, left tumor location, BRAF wild type status, KRAS mutated status, and Annexin A10 negativity. High DDR1 immunohistochemical expression was associated with shorter event free survival only. Laser capture microdissection analyses revealed that DDR1 mRNA expression was mainly attributable to adenocarcinoma compared to stromal cells. The impact of DDR1 expression on cell invasion was then evaluated by modified Boyden chamber assay using cell types with distinct mutational profiles. The invasion capacity of colon adenocarcinoma is supported by DDR1 expression. Thus, our results showed that DDR1 was highly expressed in most colon adenocarcinomas and appears as an indicator of worse event free survival.

【 授权许可】

Unknown