Screening of Antimicrobial Activities and Lipopeptide Production of Endophytic Bacteria Isolated from Vetiver Roots

Microorganisms

Sophie Slezack-Deschaumes¹  Alain Hehn¹  Yuka Munakata¹  Egon Heuson²  Matthieu Duban³  François Coutte³  Barbara Deracinois³  Théo Daboudet³ 

[1] UMR 1121, Laboratoire Agronomie et Environnement (LAE), Université de Lorraine—INRAE, F-54000 Nancy, France;Univ. Lille, CNRS, Centrale Lille, Univ. Artois, UMR 8181—UCCS—Unité de Catalyse et Chimie du Solide, F-59000 Lille, France;Université de Lille, UMRt BioEcoAgro 1158-INRAE, Équipe Métabolites Secondaires d’Origine Microbienne, Institut Charles Viollette, F-59000 Lille, France;

关键词: bacterial endophytes;    lipopeptides;    antifungal activities;    high-throughput screening;    Bacillus subtilis;    Fusarium sp.;   

DOI  :  10.3390/microorganisms10020209

来源: DOAJ

【 摘 要 】

The exploration of certain microbial resources such as beneficial endophytic microorganisms is considered a promising strategy for the discovery of new antimicrobial compounds for the pharmaceutical industries and agriculture. Thirty-one endophytic bacterial strains affiliated with Bacillus, Janthinobacterium, Yokenella, Enterobacter, Pseudomonas, Serratia, and Microbacterium were previously isolated from vetiver (Chrysopogon zizanioides (L.) Roberty) roots. These endophytes showed antifungal activity against Fusarium graminearum and could be a source of antimicrobial metabolites. In this study, in particular, using high-throughput screening, we analyzed their antagonistic activities and those of their cell-free culture supernatants against three species of Fusarium plant pathogens, a bacterial strain of Escherichia coli, and a yeast strain of Saccharomyces cerevisiae, as well as their capacity to produce lipopeptides. The results showed that the culture supernatants of four strains close to B. subtilis species exhibited antimicrobial activities against Fusarium species and E. coli. Using mass spectrometry analyses, we identified two groups of lipopeptides (surfactins and plipastatins) in their culture supernatants. Whole-genome sequencing confirmed that these bacteria possess NRPS gene clusters for surfactin and plipastatin. In vitro tests confirmed the inhibitory effect of plipastatin alone or in combination with surfactin against the three Fusarium species.

【 授权许可】

Unknown