Using cAMP Sensors to Study Cardiac Nanodomains

Journal of Cardiovascular Development and Disease

Manuela Zaccolo¹  Katharina Schleicher¹ 

[1] Department of Physiology, Anatomy and Genetics, University of Oxford, Sherrington Building, South Parks Road, Oxford OX1 3PT, UK;

关键词: 3′,5′-cyclic adenosine monophosphate;    protein kinase A;    fluorescence resonance energy transfer;    real-time imaging;    compartmentalisation;    signalling;    cardiac biology;    phosphodiesterases;    A kinase anchoring proteins;   

DOI  :  10.3390/jcdd5010017

来源: DOAJ

【 摘 要 】

3′,5′-cyclic adenosine monophosphate (cAMP) signalling plays a major role in the cardiac myocyte response to extracellular stimulation by hormones and neurotransmitters. In recent years, evidence has accumulated demonstrating that the cAMP response to different extracellular agonists is not uniform: depending on the stimulus, cAMP signals of different amplitudes and kinetics are generated in different subcellular compartments, eliciting defined physiological effects. In this review, we focus on how real-time imaging using fluorescence resonance energy transfer (FRET)-based reporters has provided mechanistic insight into the compartmentalisation of the cAMP signalling pathway and allowed for the precise definition of the regulation and function of subcellular cAMP nanodomains.

【 授权许可】

Unknown