A Multi-Region Magnetoimpedance-Based Bio-Analytical System for Ultrasensitive Simultaneous Determination of Cardiac Biomarkers Myoglobin and C-Reactive Protein

Sensors

Chong Lei¹  Yuanyuan Ding²  Pengfei Guo²  Yongsong Luo²  Zhen Yang²  Huanhuan Wang² 

[1] Department of Micro/Nano Electronics, School of electronic information and electrical engineering, Shanghai Jiao Tong University, Dongchuan Road 800, Shanghai 200240, China;School of Physics and Electronic Engineering, Xinyang Normal University, Xinyang 464000, China;

关键词: myoglobin;    C-reactive protein;    Dynabeads;    magnetoimpedance;    microfluidic device;   

DOI  :  10.3390/s18061765

来源: DOAJ

【 摘 要 】

Cardiac biomarkers (CBs) are substances that appear in the blood when the heart is damaged or stressed. Measurements of the level of CBs can be used in course of diagnostics or monitoring the state of the health of group risk persons. A multi-region bio-analytical system (MRBAS) based on magnetoimpedance (MI) changes was proposed for ultrasensitive simultaneous detection of CBs myoglobin (Mb) and C-reactive protein (CRP). The microfluidic device was designed and developed using standard microfabrication techniques for their usage in different regions, which were pre-modified with specific antibody for specified detection. Mb and CRP antigens labels attached to commercial Dynabeads with selected concentrations were trapped in different detection regions. The MI response of the triple sensitive element was carefully evaluated in initial state and in the presence of biomarkers. The results showed that the MI-based bio-sensing system had high selectivity and sensitivity for detection of CBs. Compared with the control region, ultrasensitive detections of CRP and Mb were accomplished with the detection limits of 1.0 pg/mL and 0.1 pg/mL, respectively. The linear detection range contained low concentration detection area and high concentration detection area, which were 1 pg/mL–10 ng/mL, 10–100 ng/mL for CRP, and 0.1 pg/mL–1 ng/mL, 1 n/mL–80 ng/mL for Mb. The measurement technique presented here provides a new methodology for multi-target biomolecules rapid testing.

【 授权许可】

Unknown