Svalbamides A and B, Pyrrolidinone-Bearing Lipodipeptides from Arctic Paenibacillus sp.

Marine Drugs

Yeon-Jung Lim¹  Jang-Cheon Cho¹  Sang-Jip Nam²  Seung-Il Nam³  Young-Eun Du⁴  Dong-Chan Oh⁴  Eun-Seo Bae⁴  Jong-Heon Shin⁴  Sang-Kook Lee⁴ 

[1] Department of Biological Sciences, Inha University, Incheon 22212, Korea;Department of Chemistry and Nanoscience, Ewha Womans University, Seoul 03760, Korea;Korea Polar Research Institute, Incheon 21990, Korea;Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Korea;

关键词: Paenibacillus;    Arctic;    Svalbard;    Marfey’s method;    DP4 calculation;    quinone reductase;   

DOI  :  10.3390/md19040229

来源: DOAJ

【 摘 要 】

Two new secondary metabolites, svalbamides A (1) and B (2), were isolated from a culture extract of Paenibacillus sp. SVB7 that was isolated from surface sediment from a core (HH17-1085) taken in the Svalbard archipelago in the Arctic Ocean. The combinational analysis of HR-MS and NMR spectroscopic data revealed the structures of 1 and 2 as being lipopeptides bearing 3-amino-2-pyrrolidinone, d-valine, and 3-hydroxy-8-methyldecanoic acid. The absolute configurations of the amino acid residues in svalbamides A and B were determined using the advanced Marfey’s method, in which the hydrolysates of 1 and 2 were derivatized with l- and d- forms of 1-fluoro-2,4-dinitrophenyl-5-alanine amide (FDAA). The absolute configurations of 1 and 2 were completely assigned by deducing the stereochemistry of 3-hydroxy-8-methyldecanoic acid based on DP4 calculations. Svalbamides A and B induced quinone reductase activity in Hepa1c1c7 murine hepatoma cells, indicating that they represent chemotypes with a potential for functioning as chemopreventive agents.

【 授权许可】

Unknown