Regulation of hBD-2, hBD-3, hCAP18/LL37, and Proinflammatory Cytokine Secretion by Human Milk Oligosaccharides in an Organotypic Oral Mucosal Model

Pathogens

Eva Söderling¹  Ulvi K. Gürsoy¹  Mervi Gürsoy¹  Krista Salli²  Arthur C. Ouwehand²  Johanna Hirvonen² 

[1] Department of Periodontology, Institute of Dentistry, University of Turku, Lemminkäisenkatu, 2, 20520 Turku, Finland;Global Health and Nutrition Science, IFF Health & Biosciences, Sokeritehtaantie, 20, 02460 Kantvik, Finland;

关键词: 2′-fucosyllactose;    3-fucosyllactose;    human beta defensins;    cathelicidin;    oral mucosal model;   

DOI  :  10.3390/pathogens10060739

来源: DOAJ

【 摘 要 】

Human milk oligosaccharides (HMOs), the third largest solid fraction in human milk, can modulate inflammation through Toll-like receptor signaling, but little is known about their immunomodulatory potential in the oral cavity. In this study, we determined whether the HMOs 2′-fucosyllactose (2′-FL) and 3-fucosyllactose (3-FL) regulate human-beta defensin (hBD)-2 and -3, cathelicidin (hCAP18/LL-37), and cytokine responses in human gingival cells using a three-dimensional oral mucosal culture model. The model was incubated with 0.1% or 1% 2′-FL and 3-FL, alone and in combination, for 5 or 24 h, and hBD-2, hBD-3, and hCAP18/LL-37 were analyzed by immunohistochemistry. The expression profiles of interleukin (IL)-1, IL-1RA, IL-8, and monocyte chemoattractant protein (MCP)-1 were determined by LUMINEX immunoassay. The combination of 1% 2′-FL and 1% 3-FL, and 1% 3-FL alone, for 24 h upregulated hBD-2 protein expression significantly (p < 0.001 and p = 0.016, respectively). No changes in the other antimicrobial peptides or proinflammatory cytokines were observed. Thus, 3-FL, alone and in combination with 2′-FL, stimulates oral mucosal secretion of hBD-2, without effecting a proinflammatory response when studied in an oral mucosal culture model.

【 授权许可】

Unknown