Chemical Composition of Scrophularia lucida and the Effects on Tumor Invasiveness in Vitro

Frontiers in Pharmacology

Julia Eichsteininger¹  Georg Krupitza¹  Martin Zehl²  Ali Özmen³  Chi H. Nguyen⁵  Hanspeter Kählig⁶  Liselotte Krenn⁷  Verena Lewenhofer⁷  Tobias Ledermüller⁷  Lisa Schweighofer⁷ 

[1] Clinical Institute of Pathology, Medical University of Vienna, Vienna, Austria;Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria;Department of Biology, Faculty of Science and Art, Adnan Menderes University, Aydin, Turkey;Department of Clinical Pharmacy and Diagnostics, Faculty of Life Sciences, University of Vienna, Vienna, Austria;Department of Medicine I and Comprehensive Cancer Center, Medical University of Vienna, Vienna, Austria;Department of Organic Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria;Department of Pharmacognosy, Faculty of Life Sciences, University of Vienna, Vienna, Austria;

关键词: Scrophularia lucida L.;    iridoids;    phenolic compounds;    2″-O-acetyl-homoplantaginin;    hispidulin;    intravasation;   

DOI  :  10.3389/fphar.2018.00304

来源: DOAJ

【 摘 要 】

A detannified methanolic extract of Scrophularia lucida L. attenuated the formation of cancer cell-induced circular chemorepellent induced defects (CCIDs) in the lymph endothelial cell barrier, which resemble entry ports for the intravasating tumor into the vasculature as a prerequisite for lymph node metastasis. Therefore, the composition of this extract was studied in an activity-guided approach. Since no data on the secondary metabolites of this plant were available, first phytochemical data were collected in the course of the fractionation of the extract. The study resulted in the identification of 14 substances, among them very rare iridoids, such as scrovalentinoside or koelzioside, and several flavonoids (e.g., nepitrin and homoplantaginin). One of the latter group, 2″-O-acetyl-homoplantaginin, is a new natural compound. In the most active fraction, the flavonoid hispidulin was identified as major component and the assay of the pure compound confirmed a contribution of hispidulin to the CCID-inhibitory effects of S. lucida. The activity of the two major iridoids in this assay was less compared to hispidulin.

【 授权许可】

Unknown