Eradication of Mature Bacterial Biofilms with Concurrent Improvement in Chronic Wound Healing Using Silver Nanoparticle Hydrogel Treatment

Biomedicines

Richard Bright¹  Hanif Haidari²  Sanjay Garg²  Allison J. Cowin²  Zlatko Kopecki²  Krasimir Vasilev³ 

[1] Academic Unit of STEM, University of South Australia, Mawson Lakes, SA 5095, Australia;Clinical & Health Sciences, University of South Australia, Adelaide, SA 5000, Australia;Future Industries Institute, University of South Australia, Mawson Lakes, SA 5095, Australia;

关键词: wound infection;    antibiofilm hydrogel;    AgNP hydrogel;    stimuli responsive hydrogel;    wound healing;    controlled release;   

DOI  :  10.3390/biomedicines9091182

来源: DOAJ

【 摘 要 】

Biofilm-associated infections are a major cause of impaired wound healing. Despite the broad spectrum of anti-bacterial benefits provided by silver nanoparticles (AgNPs), these materials still cause controversy due to cytotoxicity and a lack of efficacy against mature biofilms. Herein, highly potent ultrasmall AgNPs were combined with a biocompatible hydrogel with integrated synergistic functionalities to facilitate elimination of clinically relevant mature biofilms in-vivo combined with improved wound healing capacity. The delivery platform showed a superior release mechanism, reflected by high biocompatibility, hemocompatibility, and extended antibacterial efficacy. In vivo studies using the S. aureus wound biofilm model showed that the AgNP hydrogel (200 µg/g) was highly effective in eliminating biofilm infection and promoting wound repair compared to the controls, including silver sulfadiazine (Ag SD). Treatment of infected wounds with the AgNP hydrogel resulted in faster wound closure (46% closure compared to 20% for Ag SD) and accelerated wound re-epithelization (60% for AgNP), as well as improved early collagen deposition. The AgNP hydrogel did not show any toxicity to tissue and/or organs. These findings suggest that the developed AgNP hydrogel has the potential to be a safe wound treatment capable of eliminating infection and providing a safe yet effective strategy for the treatment of infected wounds.

【 授权许可】

Unknown