Enhanced production of Th1- and Th2-type antibodies and induction of regulatory T cells in mice by oral administration of Cyclopia extracts with similar phenolic composition to honeybush herbal tea

Journal of Functional Foods

Christiaan J. Malherbe¹  Elizabeth Joubert²  Hiroshi Matsuda³  Makoto Hattori³  Yutaka Miura³  Tadashi Yoshida³  Christo J.F. Muller⁴  Kazunobu Okon⁵ 

[1] Corresponding author at: Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu City, Tokyo 183-8509, Japan.;Biomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg, South Africa;Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo, Japan;Division of Animal Life Science, Tokyo University of Agriculture and Technology, Tokyo, Japan;Plant Bioactives Group, Post-Harvest and Agro-Processing Technologies Division, Agricultural Research Council, Stellenbosch, South Africa;

关键词: Antibody;    Cytokine;    Honeybush;    Regulatory T cell;    Th1 and Th2;   

DOI  :  

来源: DOAJ

【 摘 要 】

Immune-regulating activity of Cyclopia subternata and C. genistoides extracts (honeybush tea) in vitro, using a murine cell culture, merited investigation of their immune-regulating functions in vivo. Oral administration of the extracts and ovalbumin (OVA) to OVA specific-T cell receptor transgenic mice (DO11.10) significantly (P < 0.05) enhanced both Th1- and Th2-type antibody productions in the sera and generation of regulatory T cells (Treg cells) in the spleen. Demonstration of these activities in vivo confirmed those observed in vitro, suggesting that the active substances in honeybush extracts directly affected immune cells after absorption from the intestine. We demonstrated that the induced Treg cells from mice treated with C. subternata extract suppressed responses of antigen-stimulated naïve T cells when co-cultured, showing a comparable activity to naïve Treg cells. Exhibition of both immune-stimulatory and immune-regulatory activities by Cyclopia extracts in vivo supports growing consumption of Cyclopia spp. as herbal tea that can impact positively on health through the diet.

【 授权许可】

Unknown