Designing an immunosensor for detection of Brucella abortus based on coloured silica nanoparticles

Artificial Cells, Nanomedicine, and Biotechnology

Reza Shapouri¹  Mojtaba Salouti¹  Sako Mirzaii²  Bahareh Rahimian Zarif³  Arash Shams³ 

[1]Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran

[2]Department of Biochemistry, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran

[3]Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran

关键词: Brucella abortus;    colorimetric;    immunosensor;    nanoparticles;    silica;   

DOI  :  10.1080/21691401.2019.1626403

来源: DOAJ

【 摘 要 】

Brucellosis has always been a threat to the health and economics of societies. We report a new colorimetric immunoassay based on colored silica nanoparticles for detection of Brucella abortus. An immunosensor was designed based on blue-SiNPs and paramagnetic nanoparticles (PMNPs). The synthesized immunosensor was conjugated with a polyclonal antibody against B. abortus, which was activated by 1-Ethyl-3–(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) to form detection and capture probes, respectively. After adding the conjugates to the bacterial suspension, sandwich structure of PMNPs B. abortus-blue-SiNPs was formed and then separated by a magnet. The blue dye was released from the silica structure and its absorbance was measured at 670 nm with a spectrophotometer. Under optimal conditions, results showed a wide dynamic range from 1.5 × 103 to 1.5 × 108 cfu mL−1 with a detection limit of 450 cfu mL−1. The specificity of the sensor was confirmed in comparison with 5 other bacteria. Also, during the 120-days period, the complex was stable. The results suggested that it can be used in real samples (R2 = .9865). This designed colorimetric immunoassay strategy can be used as an alternative, user-friendly and on-site tool for the rapid detection of Brucella spp. compared to other common methods with high sensitivity and specificity in a short time.

【 授权许可】

Unknown