Molecular Genetic Variability of Commercial and Wild Accessions of Passion Fruit (Passiflora spp.) Targetingex Situ Conservation and Breeding

International Journal of Molecular Sciences

Ronan X. Corrêa¹  Onildo N. Jesus²  Elisa S. L. Santos³  Carlos Bernard M. Cerqueira-Silva³  João G. P. Vieira⁴  Anete P. Souza⁴  Gustavo M. Mori⁴ 

[1] Biotechnology and Genetic Center, Biological Sciences Department, State University of Santa Cruz, Ilhéus 45662-900, Brazil;Brazilian Agricultural Research Corporation, Cassava & Fruits, Cruz das Almas 44380-000, Brazil;Laboratory of Applied Molecular Genetics, Department of Exact and Natural Sciences,State University of Southwest Bahia, Itapetinga 45700-000, Brazil;Molecular Biology and Genetic Engineering Center, University of Campinas, CP 6010 Campinas, Campinas 13083-875, Brazil;

关键词: germplasm;    genetic diversity;    Passifloraceae;    molecular markers;    single sequence repeats;   

DOI  :  10.3390/ijms151222933

来源: DOAJ

【 摘 要 】

Passiflora species are distributed throughout Latin America, and Brazil and Colombia serve as the centers of diversity for this genus. We performed cross-species amplification to evaluate 109 microsatellite loci in 14 Passiflora species and estimatedthe diversity and genetic structure of Passiflora cincinnata, Passiflora setaceae and Passiflora edulis. A total of 127 accessions, including 85 accessions of P. edulis, a commercial species, and 42 accessions of 13 wild species, were examined. The cross-species amplification was effective for obtaining microsatellite loci (average cross-amplification of 70%). The average number of alleles per locus (five) was relatively low, and the average diversity ranged from 0.52 in P. cincinnata to 0.32 in P. setacea. The Bayesian analyses indicated that the P. cincinnata and P. setacea accessions were distributed into two groups, and theP. edulis accessions were distributed into five groups. Private alleles were identified, and suggestions for core collections are presented. Further collections are necessary, and the information generated may be useful for breeding and conservation.

【 授权许可】

Unknown