The Development of Cytogenetic Maps for Malaria Mosquitoes

Insects

MariaV. Sharakhova¹  VladimirN. Stegniy¹  GlebN. Artemov¹  IgorV. Sharakhov¹ 

[1] Laboratory of Ecology, Genetics and Environmental Protection, Tomsk State University, 36 Lenin Avenue, 634050 Tomsk, Russia;

关键词: cytogenetic map;    chromosome map;    malaria mosquitoes;    Anopheles;    polytene chromosomes;    Anopheles beklemishevi;    chromosome map development;    photomap;    high-pressure technique;    chromosome straightening;   

DOI  :  10.3390/insects9030121

来源: DOAJ

【 摘 要 】

Anopheline mosquitoes are important vectors of human malaria. Next-generation sequencing opens new opportunities for studies of mosquito genomes to uncover the genetic basis of a Plasmodium transmission. Physical mapping of genome sequences to polytene chromosomes significantly improves reference assemblies. High-resolution cytogenetic maps are essential for anchoring genome sequences to chromosomes as well as for studying breakpoints of chromosome rearrangements and chromatin protein localization. Here we describe a detailed pipeline for the development of high-resolution cytogenetic maps using polytene chromosomes of malaria mosquitoes. We apply this workflow to the refinement of the cytogenetic map developed for Anopheles beklemishevi.

【 授权许可】

Unknown