Genome Editing Tools in Plants

Genes

Abeer Hashem¹  Hanhong Bae²  Tufail Bashir²  Tapan Kumar Mohanta²  Elsayed Fathi Abd_Allah³ 

[1] Botany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;Department of Biotechnology, Yeungnam University, Gyeongsan 38541, Korea;Plant Production Department, College of Food and Agriculture Science, King Saud University, Riyadh 11451, Saudi Arabia;

关键词: genome editing;    homologous recombination;    Zinc finger nuclease;    TALEN;    pentatricopeptide repeat protein;    CRISPR/Cas9;    adenine base editors;    RNAi;    site-directed sequence editing;    oligonucleotide-directed mutagenesis;    cisgenesis and intragenesis;    plastid genome;    synthetic genomics;   

DOI  :  10.3390/genes8120399

来源: DOAJ

【 摘 要 】

Genome editing tools have the potential to change the genomic architecture of a genome at precise locations, with desired accuracy. These tools have been efficiently used for trait discovery and for the generation of plants with high crop yields and resistance to biotic and abiotic stresses. Due to complex genomic architecture, it is challenging to edit all of the genes/genomes using a particular genome editing tool. Therefore, to overcome this challenging task, several genome editing tools have been developed to facilitate efficient genome editing. Some of the major genome editing tools used to edit plant genomes are: Homologous recombination (HR), zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), pentatricopeptide repeat proteins (PPRs), the CRISPR/Cas9 system, RNA interference (RNAi), cisgenesis, and intragenesis. In addition, site-directed sequence editing and oligonucleotide-directed mutagenesis have the potential to edit the genome at the single-nucleotide level. Recently, adenine base editors (ABEs) have been developed to mutate A-T base pairs to G-C base pairs. ABEs use deoxyadeninedeaminase (TadA) with catalytically impaired Cas9 nickase to mutate A-T base pairs to G-C base pairs.

【 授权许可】

Unknown