PLGA-CS-PEG Microparticles for Controlled Drug Delivery in the Treatment of Triple Negative Breast Cancer Cells

Applied Sciences

Olushola Segun Odusanya¹  Sandra Musu Jusu²  Winnston Oluwole Soboyejo²  Chukwudalu Clare Nwazojie²  Vanessa Obiageli Uzonwanne³  John David Obayemi⁴  Ali Azeko Salifu⁴ 

[1] Biotechnology and Genetic Engineering Advanced Laboratory, Sheda Science and Technology Complex (SHESTCO), Abuja 900107, Nigeria;Department of Materials Science and Engineering, African University of Science and Technology, Km 10 Airport Road, Abuja 900107, Nigeria;Department of Materials Science and Engineering, Worcester Polytechnic Institute, Worcester, MA 01609, USA;Department of Mechanical Engineering, Worcester Polytechnic Institute, Worcester, MA 01609, USA;

关键词: thermodynamics;    kinetics;    drug delivery;    prodigiosin;    paclitaxel and polymeric microspheres;   

DOI  :  10.3390/app11157112

来源: DOAJ

【 摘 要 】

In this study, we explore the development of controlled PLGA-CS-PEG microspheres, which are used to encapsulate model anticancer drugs (prodigiosin (PGS) or paclitaxel (PTX)) for controlled breast cancer treatment. The PLGA microspheres are blended with hydrophilic polymers (chitosan and polyethylene glycol) in the presence of polyvinyl alcohol (PVA) that were synthesized via a water-oil-water (W/O/W) solvent evaporation technique. Chitosan (CS) and polyethylene glycol (PEG) were used as surface-modifying additives to improve the biocompatibility and reduce the adsorption of plasma proteins onto the microsphere surfaces. These PLGA-CS-PEG microspheres are loaded with varying concentrations (5 and 8 mg/mL) of PGS or PTX, respectively. Scanning electron microscopy (SEM) revealed the morphological properties while Fourier transform infrared spectroscopy (FTIR) was used to elucidate the functional groups of drug-loaded PLGA-CS-PEG microparticles. A thirty-day, in vitro, encapsulated drug (PGS or PTX) release was carried out at 37 °C, which corresponds to human body temperature, and at 41 °C and 44 °C, which correspond to hyperthermic temperatures. The thermodynamics and kinetics of in vitro drug release were also elucidated using a combination of mathematical models and the experimental results. The exponents of the Korsmeyer–Peppas model showed that the kinetics of drug release was well characterized by anomalous non-Fickian drug release. Endothermic and nonspontaneous processes are also associated with the thermodynamics of drug release. Finally, the controlled in vitro release of cancer drugs (PGS and PTX) is shown to decrease the viability of MDA-MB-231 cells. The implications of the results are discussed for the development of drug-encapsulated PLGA-CS-PEG microparticles for the controlled release of cancer drugs in treatment of triple negative breast cancer.

【 授权许可】

Unknown