Exploring the “Latin American Mediterranean” family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela

BMC Microbiology

Chyntia Carolina Díaz Acosta¹  Graciela Russomando¹  Norma Candia¹  Nora Morcillo²  Nilda J. de Romero³  Jacobus Henri De Waard⁴  Sidra E. G. Vasconcellos⁵  Harrison Magdinier Gomes⁵  Philip Noel Suffys⁵  Marcia de Berrêdo Pinho Moreira⁶  Viviana Ritacco⁷ 

[1] Departamento de Biología Molecular y Biotecnología. Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción;Instituto Nacional de Enfermedades Respiratorias Emilio Coni;Laboratorio Central de Salud Pública, MSP y BS;Laboratorio de Tuberculosis, Instituto de Biomedicina;Laboratório de Biologia Molecular aplicada às Micobactérias, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz;Laboratório de Microbiologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz;Servicio de Micobacterias, Instituto Nacional de Enfermedades Infecciosas, ANLIS “Carlos G. Malbran”;

关键词: Mycobacterium tuberculosis;    LAM;    Genotyping;    Paraguay;    South-America;   

DOI  :  10.1186/s12866-019-1479-6

来源: DOAJ

【 摘 要 】

Abstract Background The Latin American & Mediterranean (LAM) spoligotype family is one of the most successful genotype of Mycobacterium tuberculosis worldwide and particularly prevalent in South-America. Within this family, a sublineage named Region of Difference Rio (RDRio) was reported initially in Brazil and is characterized by a genomic deletion of about 26.3 kb. This lineage seems to show a specific adaptation to the Euro-Latin American population. In this context, we sought to evaluate the LAM family and the presence of the RDRio genotype in samples from three Latin American countries including Paraguay, Venezuela and Argentina. To detect LAM strains reliably we applied a typing scheme using spoligotyping, 12 loci MIRU-VNTR, the Ag85C103 SNP and the regions of difference RDRio and RD174. IS6110-RFLP results were also used when available. Results Genotyping of 413 M. tuberculosis isolates from three Latin-American countries detected LAM (46%) and the ill-defined T clade (16%) as the most frequent families. The highest clustering rate was detected in the sample population from the city of Caracas in Venezuela. We observed considerable differences in the presence of the RDRio lineage, with high frequency in Caracas-Venezuela (55%) and low frequency in Buenos Aires-Argentina (11%) and Paraguay (10%). The molecular markers (RD174, Ag85C103, MIRU02-MIRU40 signature) of the RDRio lineage were essentially confirmed. For the LAM family, the most polymorphic loci were MIRU40, MIRU31, MIRU10, MIRU26, MIRU16 and the least polymorphic MIRU24, MIRU20, MIRU04, MIRU23. Conclusions Our results suggest a differential adaptation of LAM-sublineages in neighboring populations and that RDRio strains spread regionally with different rates of distribution. The Ag85C SNP and RDs (RD174, RDRio) tested in this study can in fact facilitate molecular epidemiological studies of LAM strains in endemic settings and low-income countries.

【 授权许可】

Unknown