Determination of Homocysteine in Urine and Saliva by Microchip Electrophoresis

Hungarian Journal of Industry and Chemistry

Hradski Jasna¹  Troška Peter¹  Mandžáková Anna¹  Masár Marián¹  Ševčík Juraj² 

[1]Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina CH-2,Bratislava, Slovak Republic

[2]Department of Analytical Chemistry, Faculty of Science, Palacký University Olomouc,Olomouc, Czech Republic

关键词: homocysteine;    body fluids;    microchip electrophoresis;    solid-phase microextraction;   

DOI  :  10.1515/hjic-2018-0010

来源: DOAJ

【 摘 要 】

An online combination of isotachophoresis (ITP) and capillary zone electrophoresis (CZE) was employed on a microchip with a column-coupling technology for the determination of homocysteine in various body fluids. ITP with its high concentration ability was used as a sample pretreatment and injection technique for CZE, which facilitated the rapid and sensitive determination of homocysteine. The resolution of the analyte from other constituents present in real complex samples was enhanced by discrete spacers, which were added to the injected sample. A solid-phase microextraction (SPME) pretreatment technique based on silver- and barium-form resins was used prior to the ITP-CZE analysis to remove high concentrations of chloride and sulfate naturally present in the analyzed samples. The combination of the micropretreatment and microelectrophoresis techniques facilitated the determination of trace concentrations of homocysteine in samples of urine and saliva

【 授权许可】

Unknown