Challenges in Quantifying Cytosine Methylation in the HIV Provirus

mBio

Antoine Chaillon¹  Christina Huynh¹  Sara Gianella¹  Sarah A. LaMere¹  Davey M. Smith¹ 

[1] Department of Medicine, University of California San Diego, La Jolla, California, USA;

关键词: HIV latency;    cytosine methylation;    epigenetic silencing;    non-CpG methylation;   

DOI  :  10.1128/mBio.02268-18

来源: DOAJ

【 摘 要 】

ABSTRACT DNA methylation is an epigenetic mechanism most commonly associated with transcriptional repression. While it is clear that DNA methylation can silence HIV proviral expression in in vitro latency models, its correlation with HIV persistence and expression in vivo is ambiguous, particularly in persons living with HIV (PLWH) receiving antiretroviral therapy (ART). Several factors potentially contribute to discrepancies between results in the literature, including differences in integration sites, functional proviral load, sampling bias, and stochastic PCR amplification. Recent studies into genomic features of cytosine methylation sites in mammalian genes offer potentially significant insights into this mechanism. Here, we discuss the importance of these factors in the context of the HIV.

【 授权许可】

Unknown