Seed Cryopreservation, Germination, and Micropropagation of Eastern Turkeybeard, Xerophyllum asphodeloides (L.) Nutt.: A Threatened Species from the Southeastern United States

Plants

Ron O. Determann¹  Jenifer M. Cruse-Sanders¹  Shannon Johnson²  Kylie Bucalo²  Gerald S. Pullman³  Alex B. Costa³  Princy Kuriakose³  Michelle Issac³  Stacie Leung³  Will L. Rogers⁴  Jonathan M. Stober⁵ 

[1] Department of Conservation Research, Atlanta Botanical Garden, 1345 Piedmont Ave., NE, Atlanta, GA 30309, USA;Renewable Bioproducts Institute, Georgia Institute of Technology (Formerly the Institute of Paper Science and Technology), 500 10th Street NW, Atlanta, GA 30332, USA;School of Biology, Georgia Institute of Technology, Atlanta, GA 30332, USA;State Botanical Garden of Georgia, 2450 Milledge Avenue, Athens, GA 30605, USA;US Forest Service, USDA, Talladega National Forest, Shoal Creek Ranger Dist, Heflin, AL 36264, USA;

关键词: conservation;    cryopreservation;    endangered species;    micropropagation;    shoot culture;    Xerophyllum asphodeloides;   

DOI  :  10.3390/plants10071462

来源: DOAJ

【 摘 要 】

Xerophyllum asphodeloides (Xerophyllaceae), known as eastern turkeybeard, is an herbaceous perennial found in eastern North America. Due to decline and destruction of its habitat, several states rank X. asphodeloides as “Imperiled” to “Critically Imperiled”. Protocols for seed cryopreservation, in vitro germination, sustainable shoot micropropagation, shoot establishment in soil, and seed germination are presented. Seeds from two tested sources were viable after 20 months of cryopreservation. Germination of isolated embryos in vitro was necessary to overcome strong seed dormancy. Shoot multiplication and elongation occurred on ½ MS medium without PGRs. Shoots rooted in vitro without PGRs or with 0.5 mg/L NAA or after NAA rooting powder treatment and placement in potting mix. When planted in wet, peaty soil mixes, shoots grew for two months and then declined. When planted in a drier planting mix containing aged bark, most plants continued growth. In the field, plant survival was 73% after three growing seasons. Safeguarding this species both ex situ and in situ is possible and offers a successful approach to conservation. Whole seeds germinated after double dormancy was overcome by incubation under warm moist conditions for 12 weeks followed by 12 weeks cold at 4 °C and then warm.

【 授权许可】

Unknown