Low Intensity Pulsed Ultrasound Prevents Recurrent Ischemic Stroke in a Cerebral Ischemia/Reperfusion Injury Mouse Model via Brain-derived Neurotrophic Factor Induction

International Journal of Molecular Sciences

Cheng-Tien Wu¹  Ting-Hua Yang²  Lin-Hwa Long³  Chang-Mu Chen³  Siao-Syun Guan⁴  Yao-Pang Chung⁵  Man-Chih Chen⁵  Shing-Hwa Liu⁵ 

[1] Department of Nutrition, China Medical University, Taichung 40402, Taiwan;Department of Otolaryngology, College of Medicine and Hospital, National Taiwan University, Taipei 10051, Taiwan;Division of Neurosurgery, Department of Surgery, College of Medicine and Hospital, National Taiwan University, Taipei 10051, Taiwan;Institute of Nuclear Energy Research, Atomic Energy Council, Taoyuan 32546, Taiwan;Institute of Toxicology, College of Medicine, National Taiwan University, Taipei 10051, Taiwan;

关键词: apoptosis;    brain-derived neurotrophic factor;    ischemic stroke;    low-intensity pulsed ultrasound;    stroke recurrence;   

DOI  :  10.3390/ijms20205169

来源: DOAJ

【 摘 要 】

The incidence of stroke recurrence is still higher despite the advanced progression of therapeutic treatment and medical technology. Low intensity pulsed ultrasound (LIPUS) has been demonstrated to possess therapeutic effects on neuronal diseases and stroke via brain-derived neurotrophic factor (BDNF) induction. In this study, we hypothesized that LIPUS treatment possessed therapeutic benefits for the improvement of stroke recurrence. Adult male C57BL/6J mice were subjected to a middle cerebral artery occlusion (MCAO) surgery and then followed to secondary MCAO surgery as a stroke recurrence occurred after nine days from the first MCAO. LIPUS was administered continuously for nine days before secondary MCAO. LIPUS treatment not only decreased the mortality but also significantly moderated neuronal function injury including neurological score, motor activity, and brain pathological score in the recurrent stroke mice. Furthermore, the administration of LIPUS attenuated the apoptotic neuronal cells and increased Bax/Bcl-2 protein expression ratio and accelerated the expression of BDNF in the brain of the recurrent stroke mice. Taken together, these results demonstrate for the first time that LIPUS treatment arouses the expression of BDNF and possesses a therapeutic benefit for the improvement of stroke recurrence in a mouse model. The neuroprotective potential of LIPUS may provide a useful strategy for the prevention of a recurrent stroke.

【 授权许可】

Unknown