Evolution of A bHLH Interaction Motif

International Journal of Molecular Sciences

Meike Burow¹  BirtheB. Kragelund²  PeterS. Millard² 

[1] DynaMo Center, Department of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark;The Linderstrøm-Lang Centre for Protein Science, Department of Biology, University of Copenhagen, DK-2200 Copenhagen N, Denmark;

关键词: MYB;    bHLH;    MYC;    transcription factor;    short linear motif (SLiM);    intrinsically disordered protein (IDP);   

DOI  :  10.3390/ijms22010447

来源: DOAJ

【 摘 要 】

Intrinsically disordered proteins and regions with their associated short linear motifs play key roles in transcriptional regulation. The disordered MYC-interaction motif (MIM) mediates interactions between MYC and MYB transcription factors in Arabidopsis thaliana that are critical for constitutive and induced glucosinolate (GLS) biosynthesis. GLSs comprise a class of plant defense compounds that evolved in the ancestor of the Brassicales order. We used a diverse set of search strategies to discover additional occurrences of the MIM in other proteins and in other organisms and evaluate the findings by means of structural predictions, interaction assays, and biophysical experiments. Our search revealed numerous MIM instances spread throughout the angiosperm lineage. Experiments verify that several of the newly discovered MIM-containing proteins interact with MYC TFs. Only hits found within the same transcription factor family and having similar characteristics could be validated, indicating that structural predictions and sequence similarity are good indicators of whether the presence of a MIM mediates interaction. The experimentally validated MIMs are found in organisms outside the Brassicales order, showing that MIM function is broader than regulating GLS biosynthesis.

【 授权许可】

Unknown