Polysaccharides from Dendrobium officinale inhibit proliferation of osteosarcoma cells and enhance cisplatin-induced apoptosis

Journal of Functional Foods

Chenxing Liu¹  Gang Wei²  Yunrong Li³  Zhouxi Lei⁴  Shengchang Tao⁴  Shuna Duan⁴  Xiaofeng Zhang⁴  Zhiyao Ren⁴  Jiahui Huang⁴  Shangping Xing⁴ 

[1] Shaoguan Institute of Danxia Dendrobium Officinale, Shaoguan 512005, China;Guangxi International Zhuang Medicine Hospital, The Affiliated International Zhuang Medicine Hospital, Nanning 530201, China;Guangzhou Baiyunshan Chenliji Pharmaceutical Co., Ltd., Guangzhou 510006, China;School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;

关键词: Dendrobium officinale polysaccharide;    Mannose;    Osteosarcoma;    Cisplatin;    Proliferation;    Apoptosis;   

DOI  :  

来源: DOAJ

【 摘 要 】

Dendrobium officinale polysaccharides (DOPs) are carbohydrates predominantly composed of mannose (M1) and glucose, and are known to exhibit antitumour activities. Here, the antitumour properties of carbohydrates including monosaccharide M1, oligosaccharide mannohexaose (M6), and DOPs were investigated. Polysaccharides DOP122, DOP20, DOP8, and DOP2 with molecular weights of 1224.54, 200.99, 80.32, and 24.89 kDa, respectively, were obtained from Dendrobium officinale. Among M1, M6, and DOP122-DOP2, DOP20 exerted the strongest inhibitory effect on the proliferation of osteosarcoma (OS) U2OS and Saos-2 cells. When combined with cisplatin (DDP), DOP20 showed a synergistic inhibition of proliferation. Moreover, DOP20 enhanced DDP-induced cell apoptosis mediated by the mitochondrial pathway by up-regulating P53, Bax, and Bak expression; down-regulating Bcl-2 and Mcl-1 expression; and increasing Cleaved caspase9/Caspase9, Cleaved caspase3/Caspase3, and Cleaved PARP/PARP ratios. These findings confirmed that carbohydrate drugs, especially polysaccharides, used in the treatment of tumours need to be validated in terms of a suitable molecular weight.

【 授权许可】

Unknown