SSR genetic linkage map construction of pea (Pisum sativum L.) based on Chinese native varieties

Crop Journal

Xuelian Sun¹  Fang Wang²  Jianping Guan³  Junjie Hao³  Xuxiao Zong⁴  Tao Yang⁴  Xiaoyan Zhang⁴  Rebecca Ford⁵  Junye Jiang⁵ 

[1] Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China;Department of Agriculture and Food Systems, Melbourne School of Land and Environment, The University of Melbourne, Melbourne, Victoria 3010, Australia;Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China;National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, 100081, China;Qingdao Academy of Agricultural Sciences, Qingdao 266100, China;

关键词: Pea;    SSR markers;    Genetic linkage map;    Marker-assisted breeding;   

DOI  :  doi:10.1016/j.cj.2014.03.004

来源: DOAJ

【 摘 要 】

Simple sequence repeat (SSR) markers have previously been applied to linkage mapping of the pea (Pisum sativum L.) genome. However, the transferability of existing loci to the molecularly distinct Chinese winter pea gene pool was limited. A novel set of pea SSR markers was accordingly developed. Together with existing SSR sequences, the genome of the G0003973 (winter hardy) × G0005527 (cold sensitive) cross was mapped using 190 F2 individuals. In total, 157 SSR markers were placed in 11 linkage groups with an average interval of 9.7 cM and total coverage of 1518 cM. The novel markers and genetic linkage map will be useful for marker-assisted pea breeding.

【 授权许可】

Unknown