Whole-Body Cryotherapy Increases the Activity of Nitric Oxide Synthase in Older Men

Biomolecules

Joanna Gradek¹  Jadwiga Szymura²  Justyna Kusmierczyk³  Magdalena Wiecek³  Zbigniew Szygula⁴ 

[1] Department of Athletics, Faculty of Physical Education and Sport, University of Physical Education in Kraków, 31-571 Kraków, Poland;Department of Clinical Rehabilitation, Faculty of Motor Rehabilitation, University of Physical Education in Kraków, 31-571 Kraków, Poland;Department of Physiology and Biochemistry, Institute of Biomedical Sciences, Faculty of Physical Education and Sport, University of Physical Education in Kraków, 31-571 Kraków, Poland;Department of Sports Medicine and Human Nutrition, Institute of Biomedical Sciences, Faculty of Physical Education and Sport, University of Physical Education in Kraków, 31-571 Kraków, Poland;

关键词: whole-body cryostimulation;    aging;    oxidative stress;    nitric oxide synthase;    vasodilatation;    endurance athletes;   

DOI  :  10.3390/biom11071041

来源: DOAJ

【 摘 要 】

Aging causes oxidative stress, endothelial dysfunction and a reduction in the bioavailability of nitric oxide. The study aim was to determine whether, as a result of repeated whole-body exposure to cryogenic temperature (3 min −130 °C), there is an increase of inducible nitric oxide synthase (iNOS) concentration in senior subjects (59 ± 6 years), and if this effect is stronger in athletes. In 10 long-distance runners (RUN) and 10 untraining (UTR) men, 24 whole-body cryotherapy (WBC) procedures were performed. Prior to WBC, after 12th and 24th treatments and 7 days later, the concentration of iNOS, asymmetric dimethylarginine (ADMA), 3-nitrotyrosine (3-NTR), homocysteine (HCY), C-reactive protein (CRP) and interleukins such as: IL-6, IL-1β, IL-10 were measured. In the RUN and UTR groups, after 24 WBC, iNOS concentration was found to be comparable and significantly higher (F = 5.95, p < 0.01) (large clinical effect size) compared to before 1st WBC and after 12th WBC sessions. There were no changes in the concentration of the remaining markers as a result of WBC (p > 0.05). As a result of applying 24 WBC treatments, using the every-other-day model, iNOS concentration increased in the group of older men, regardless of their physical activity level. Along with this increase, there were no changes in nitro-oxidative stress or inflammation marker levels.

【 授权许可】

Unknown