Spontaneous Regeneration of Plantlets Derived from Hairy Root Cultures of Lopezia racemosa and the Cytotoxic Activity of Their Organic Extracts

Plants

Alfonso Leija-Salas¹  Norma Elizabeth Moreno-Anzúrez²  Jaime Tortoriello-García³  Edgar R. Díaz-García³  Pilar Nicasio-Torres³  Jesús Arellano-García⁴  Norely Vargas-Morales⁴  Irene Perea-Arango⁴  Susana Valencia-Díaz⁴  María Del Carmen Gutiérrez-Villafuerte⁴  Janeth Téllez-Román⁵ 

[1] Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Av. Universidad s/n Col. Chamilpa C.P., Cuernavaca 62210, Morelos, Mexico;Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Carretera Yautepec-Jojutla Km 6, Calle CEPROBI No. 8, Colonia San Isidro C.P., Yautepec 62731, Morelos, Mexico;Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1 Col. Centro C.P., Xochitepec 62790, Morelos, Mexico;Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001 Col. Chamilpa C.P., Cuernavaca 62209, Morelos, Mexico;Facultad de Medicina, Universidad Autónoma del Estado de Morelos, Iztatzíhuatl esq. Leñeros s/n Col. Los Volcanes C.P., Cuernavaca 62350, Morelos, Mexico;

关键词: organogenesis;    somatic embryogenesis;    Lopezia racemosa;    genetic transformation;    cancer cell lines;   

DOI  :  10.3390/plants11020150

来源: DOAJ

【 摘 要 】

A histological analysis was performed with the aim of elucidating the spontaneous regeneration process of the hairy root lines LRT 2.3 and LRT 6.4, derived from Lopezia racemosa leaf explants and genetically transformed with the Agrobacterium rhizogenes strain ATCC15834/pTDT. The analysis showed both lines regenerate via indirect somatic embryogenesis; LRT 6.4 also regenerated by direct organogenesis. The morphogenic characteristics of the regenerated plantlets from both lines showed the typical characteristics, described previously, including a higher number of axillary shoot formation, short internodes, and plagiotropic roots compared with wild-type seedlings. The regeneration process occurred without the addition of plant growth regulators and was linked to the sucrose concentration in the culture medium. Reducing the sucrose concentration from 3% to 2%, 1%, and 0.5% increased the regeneration rate in LRT 6.4; the effect was less pronounced in LRT 2.3. The cytotoxic activity of different organic extracts obtained from roots and shoots were evaluated in the cancer cell lines HeLa (cervical carcinoma), HCT-15 (colon adenocarcinoma), and OVCAR (ovary carcinoma). The hexane and dichloromethane extracts from roots of both lines showed cytotoxic activity against the HeLa cell line. Only the dichloromethane extract from the roots of PLRT 2.3 showed cytotoxic activity against the OVCAR cell line. None of the methanol extracts showed cytotoxic activity, nor the shoot extracts from any solvent.

【 授权许可】

Unknown