Co-Inoculation of Bacillus velezensis Strain S141 and Bradyrhizobium Strains Promotes Nodule Growth and Nitrogen Fixation

Microorganisms

Takahiko Kondo¹  Ken-ichi Yoshida¹  Kosei Tanaka²  Neung Teaumroong³  Nantakorn Boonkerd³  Panlada Tittabutr³  Surachat Sibponkrung³ 

[1]Graduate School of Science, Technology and Innovation, Kobe University, Kobe 657-8501, Japan

[2]Organization of Advanced Science and Technology, Kobe University, Kobe 657-8501, Japan

[3]School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand

关键词: PGPR;    Bradyrhizobium;    Co-inoculation;    Soybean;    Nodulation;   

DOI  :  10.3390/microorganisms8050678

来源: DOAJ

【 摘 要 】

The objective of this research was to evaluate the PGPR effect on nodulation and nitrogen-fixing efficiency of soybean (Glycine max (L.) Merr.) by co-inoculation with Bradyrhizobium diazoefficiens USDA110. Co-inoculation of Bacillus velezensis S141 with USDA110 into soybean resulted in enhanced nodulation and N2-fixing efficiency by producing larger nodules. To understand the role of S141 on soybean and USDA110 symbiosis, putative genes related to IAA biosynthesis were disrupted, suggesting that co-inoculation of USDA110 with S141ΔyhcX reduces the number of large size nodules. It was revealed that yhcX may play a major role in IAA biosynthesis in S141 as well as provide a major impact on soybean growth promotion. The disruption of genes related to cytokinin biosynthesis and co-inoculation of USDA110 with S141ΔIPI reduced the number of very large size nodules, and it appears that IPI might play an important role in nodule size of soybean–Bradyrhizobium symbiosis. However, it was possible that not only IAA and cytokinin but also some other substances secreted from S141 facilitate Bradyrhizobium to trigger bigger nodule formation, resulting in enhanced N2-fixation. Therefore, the ability of S141 with Bradyrhizobium co-inoculation to enhance soybean N2-fixation strategy could be further developed for supreme soybean inoculants.

【 授权许可】

Unknown