Age-dependent alterations in the presynaptic active zone in a Drosophila model of Alzheimer's Disease

Neurobiology of Disease

Fu-De Huang¹  Xiao-Jiang Sun²  Jian-Kang Huang²  Jiang-Xiu Tan³  Ping-Li Ma³  Su-Ying Ji³  Xiao-Liang Zhao³ 

[1] Corresponding authors.;Department of Neurology, The Sixth People's Hospital affiliated to Shanghai Jiaotong University, Shanghai 200233, China;Institute of Neuroscience and State Key Laboratory of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;

关键词: Beta amyloid;    Synaptic vesicle release probability;    VGCC;    Bruchpilot;    Presynaptic active zone;    Drosophila;   

DOI  :  

来源: DOAJ

【 摘 要 】

The accumulation of beta amyloid (Aβ) can cause synaptic impairments, but the characteristics and mechanisms of the synaptic impairment induced by the accumulation of Aβ in Alzheimer's disease (AD) remain unclear. In identified single neurons in a newly developed Drosophila AD model, in which Aβ accumulates intraneuronally, we found an age-dependent reduction in the synaptic vesicle release probability that was associated with a decrease in the density of presynaptic calcium channel clusters and an increase in the presynaptic and postsynaptic contact length. Moreover, these alterations occurred in the absence of presynaptic bouton loss. In addition, we found that Aβ expression also produced an age-dependent decrease in the amount of Bruchpilot (Brp), which plays an important role in controlling Ca2+ channel clustering and synaptic vesicle release in the presynaptic active zone. Our study indicates that the chronic accumulation of intraneuronal Aβ can induce functional and structural changes in the presynaptic active zone prior to a loss of presynaptic buttons in the same neuron.

【 授权许可】

Unknown