Clathrin-mediated endocytosis cooperates with bulk endocytosis to generate vesicles

iScience

Justin W. Taraska¹  Oleg Shupliakov²  Uri Ashery³  Agila Somasundaram⁴  Ling-Gang Wu⁴  Wonchul Shin⁵  Seth Villareal⁵  Chung Yu Chan⁵  Gianvito Arpino⁵  Lihao Ge⁵ 

[1] Institute of Translational Biomedicine, St Petersburg State University, St Petersburg, Russia;Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden;Life Science Faculty, Sagol School of Neuroscience, Tel Aviv University, Tel Aviv, Israel;National Heart, Lung, and Blood Institute, Bethesda, MD, USA;National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA;

关键词: Biological sciences;    Neuroscience;    Endocrine regulation;    Cell biology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Clathrin-mediated endocytosis, the most prominent endocytic mode, is thought to be generated primarily from relatively flat patches of the plasma membrane. By employing conventional and platinum replica electron microscopy and super-resolution STED microscopy in neuroendocrine chromaffin cells, we found that large Ω-shaped or dome-shaped plasma membrane invaginations, previously thought of as the precursor of bulk endocytosis, are primary sites for clathrin-coated pit generation after depolarization. Clathrin-coated pits are more densely packed at invaginations rather than flat membranes, suggesting that invaginations are preferred sites for clathrin-coated pit formation, likely because their positive curvature facilitates coated-pit formation. Thus, clathrin-mediated endocytosis closely collaborates with bulk endocytosis to enhance endocytic capacity in active secretory cells. This direct collaboration between two classically independent endocytic pathways is of broad importance given the central role of both clathrin-mediated endocytosis and bulk endocytosis in neurons, endocrine cells, immune cells, and many other cell types throughout the body.

【 授权许可】

Unknown