Direct translation of climbing fiber burst-mediated sensory coding into post-synaptic Purkinje cell dendritic calcium

eLife

Seung Ha Kim¹  Sun Kwang Kim¹  Yong Gyu Kim²  Chang-Eop Kim³  Changhyeon Ryu⁴  Seung-Eon Roh⁵  Sang Jeong Kim⁵  Paul F Worley⁵ 

[1] Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Republic of Korea;Department of Neuroscience, School of Medicine, Johns Hopkins University, Baltimore, United States;Department of Physiology, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea;Neuroscience Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea;Department of Physiology, Seoul National University College of Medicine, Seoul, Republic of Korea;

关键词: climbing fiber;    climbing fiber burst;    purkinje cell calcium;    cerebellum;    sensory coding;    two-photon calcium imaging;   

DOI  :  10.7554/eLife.61593

来源: DOAJ

【 摘 要 】

Climbing fibers (CFs) generate complex spikes (CS) and Ca2+ transients in cerebellar Purkinje cells (PCs), serving as instructive signals. The so-called 'all-or-none' character of CSs has been questioned since the CF burst was described. Although recent studies have indicated a sensory-driven enhancement of PC Ca2+ signals, how CF responds to sensory events and contributes to PC dendritic Ca2+ and CS remains unexplored. Here, single or simultaneous Ca2+ imaging of CFs and PCs in awake mice revealed the presynaptic CF Ca2+ amplitude encoded the sensory input’s strength and directly influenced post-synaptic PC dendritic Ca2+ amplitude. The sensory-driven variability in CF Ca2+ amplitude depended on the number of spikes in the CF burst. Finally, the spike number of the CF burst determined the PC Ca2+ influx and CS properties. These results reveal the direct translation of sensory information-coding CF inputs into PC Ca2+, suggesting the sophisticated role of CFs as error signals.

【 授权许可】

Unknown