Diversity and N2O Production Potential of Fungi in an Oceanic Oxygen Minimum Zone

Journal of Fungi

DavidL. Valentine¹  Xuefeng Peng¹ 

[1] Marine Science Institute, University of California, Santa Barbara, CA 93106, USA;

关键词: marine fungi;    oxygen minimum zone;    nitrous oxide;    diversity;    ¹⁵N tracer;    size-fractioned;   

DOI  :  10.3390/jof7030218

来源: DOAJ

【 摘 要 】

Fungi in terrestrial environments are known to play a key role in carbon and nitrogen biogeochemistry and exhibit high diversity. In contrast, the diversity and function of fungi in the ocean has remained underexplored and largely neglected. In the eastern tropical North Pacific oxygen minimum zone, we examined the fungal diversity by sequencing the internal transcribed spacer region 2 (ITS2) and mining a metagenome dataset collected from the same region. Additionally, we coupled ¹⁵N-tracer experiments with a selective inhibition method to determine the potential contribution of marine fungi to nitrous oxide (N₂O) production. Fungal communities evaluated by ITS2 sequencing were dominated by the phyla Basidiomycota and Ascomycota at most depths. However, the metagenome dataset showed that about one third of the fungal community belong to early-diverging phyla. Fungal N₂O production rates peaked at the oxic–anoxic interface of the water column, and when integrated from the oxycline to the top of the anoxic depths, fungi accounted for 18–22% of total N₂O production. Our findings highlight the limitation of ITS-based methods typically used to investigate terrestrial fungal diversity and indicate that fungi may play an active role in marine nitrogen cycling.

【 授权许可】

Unknown