Enhanced Radiosensitization for Cancer Treatment with Gold Nanoparticles through Sonoporation

International Journal of Molecular Sciences

Churng-Ren Chris Wang¹  Jason Chia-Hsien Cheng²  Shao-Lun Lu²  Wei-Wen Liu²  Lien-Chieh Lin²  Pai-Chi Li² 

[1] Department of Chemistry and Biochemistry, National Chung-Cheng University, Chia-Yi 621301, Taiwan;Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei 10617, Taiwan;

关键词: sonoporation;    microbubbles;    gold nanoparticles;    cavitation;    radiotherapy;    radiosensitization;   

DOI  :  10.3390/ijms21218370

来源: DOAJ

【 摘 要 】

We demonstrate the megavoltage (MV) radiosensitization of a human liver cancer line by combining gold-nanoparticle-encapsulated microbubbles (AuMBs) with ultrasound. Microbubbles-mediated sonoporation was administered for 5 min, at 2 h prior to applying radiotherapy. The intracellular concentration of gold nanoparticles (AuNPs) increased with the inertial cavitation of AuMBs in a dose-dependent manner. A higher inertial cavitation dose was also associated with more DNA damage, higher levels of apoptosis markers, and inferior cell surviving fractions after MV X-ray irradiation. The dose-modifying ratio in a clonogenic assay was 1.56 ± 0.45 for a 10% surviving fraction. In a xenograft mouse model, combining vascular endothelial growth factor receptor 2 (VEGFR2)-targeted AuMBs with sonoporation significantly delayed tumor regrowth. A strategy involving the spatially and temporally controlled release of AuNPs followed by clinically utilized MV irradiation shows promising results that make it worthy of further translational investigations.

【 授权许可】

Unknown