Automated Cytogenetic Biodosimetry at Population-Scale

Radiation

Farrah Norton¹  Joan H. M. Knoll²  Yanxin Li²  Ben C. Shirley²  Ngoc-Duy Pham³  Peter K. Rogan⁴  Eliseos J. Mucaki⁴  Ed Waller⁵  Ruth C. Wilkins⁶  Olga Sevriukova⁷ 

[1] Canadian Nuclear Laboratories, Radiobiology and Health, Chalk River, ON K0J 1J0, Canada;CytoGnomix Inc., London, ON N5X 3X5, Canada;Dalat Nuclear Research Institute, Dalat 670000, Vietnam;Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON N6A 5C1, Canada;Energy Systems and Nuclear Science, University of Ontario Institute of Technology, Oshawa, ON L1G 0C5, Canada;Health Canada, Consumer and Clinical Radiation Protection Bureau, Ottawa, ON K1A 1C1, Canada;Radiation Protection Centre, LT-08352 Vilnius, Lithuania;

关键词: humans;    metaphase;    censuses;    population density;    triage;    chromosomes;   

DOI  :  10.3390/radiation1020008

来源: DOAJ

【 摘 要 】

The dicentric chromosome (DC) assay accurately quantifies exposure to radiation; however, manual and semi-automated assignment of DCs has limited its use for a potential large-scale radiation incident. The Automated Dicentric Chromosome Identifier and Dose Estimator (ADCI) software automates unattended DC detection and determines radiation exposures, fulfilling IAEA criteria for triage biodosimetry. This study evaluates the throughput of high-performance ADCI (ADCI-HT) to stratify exposures of populations in 15 simulated population scale radiation exposures. ADCI-HT streamlines dose estimation using a supercomputer by optimal hierarchical scheduling of DC detection for varying numbers of samples and metaphase cell images in parallel on multiple processors. We evaluated processing times and accuracy of estimated exposures across census-defined populations. Image processing of 1744 samples on 16,384 CPUs required 1 h 11 min 23 s and radiation dose estimation based on DC frequencies required 32 sec. Processing of 40,000 samples at 10 exposures from five laboratories required 25 h and met IAEA criteria (dose estimates were within 0.5 Gy; median = 0.07). Geostatistically interpolated radiation exposure contours of simulated nuclear incidents were defined by samples exposed to clinically relevant exposure levels (1 and 2 Gy). Analysis of all exposed individuals with ADCI-HT required 0.6–7.4 days, depending on the population density of the simulation.

【 授权许可】

Unknown