A Murine Dopamine Neuron-Specific cDNA Library and Microarray: Increased COXI Expression during Methamphetamine Neurotoxicity

Neurobiology of Disease

Yulan Piao¹  Kevin G. Becker¹  Robert Wersto¹  Minoru S. Ko¹  Ora Dillon-Carter²  Christopher Cheadle²  George A. Ricaurte²  Daniel L. Rowley²  George J. Kargul³  David M. Donovan³  William H. Wood, III³  Magdalena Juhaszova³  Tao Xie⁴  Marquis P. Vawter⁴  William J. Freed⁴  Francis J. Chrest⁴  Li Zhou⁵  Tanya Barrett⁵  Una D. McCann⁵  Meng K. Lim⁵ 

[1]Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224-6825

[2]Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205

[3]Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205

[4]Laboratory of Genetics, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive

[5]Research Resources Branch, Intramural Research Program, National Institute on Aging, 5600 Nathan Shock Drive

关键词: dopamine;    cDNA library;    cDNA microarray;    methamphetamine;    development;    gene expression profile;   

DOI  :  

来源: DOAJ

【 摘 要 】

Due to brain tissue heterogeneity, the molecular genetic profile of any neurotransmitter-specific neuronal subtype is unknown. The purpose of this study was to purify a population of dopamine neurons, construct a cDNA library, and generate an initial gene expression profile and a microarray representative of dopamine neuron transcripts. Ventral mesencephalic dopamine neurons were purified by fluorescent-activated cell sorting from embryonic day 13.5 transgenic mice harboring a 4.5-kb rat tyrosine hydroxylase promoter–lacZ fusion. Nine-hundred sixty dopamine neuron cDNA clones were sequenced and arrayed for use in studies of gene expression changes during methamphetamine neurotoxicity. A neurotoxic dose of methamphetamine produced a greater than twofold up-regulation of the mitochondrial cytochrome c oxidase polypeptide I transcript from adult mouse substantia nigra at 12 h posttreatment. This is the first work to describe a gene expression profile for a neuronal subtype and to identify gene expression changes during methamphetamine neurotoxicity.

【 授权许可】

Unknown