Neuronal Differentiation from Induced Pluripotent Stem Cell-Derived Neurospheres by the Application of Oxidized Alginate-Gelatin-Laminin Hydrogels

Biomedicines

Rainer Detsch¹  AldoR. Boccaccini¹  Thomas Distler¹  Stephan Rütten²  Ellen Fritsche³  Ines Lauria³  Farina Bendt³  Julia Kapr³  ClemensM. Sauter³ 

[1] Department of Materials Science and Engineering, Institute Biomaterials, University of Erlangen-Nuremberg, Cauerstr. 6, 91058 Erlangen, Germany;Electron Microscopy Facility, Institute of Pathology, RWTH Aachen University Hospital, Pauwelsstrasse 30, 52074 Aachen, Germany;IUF-Leibniz Research Institute for Environmental Medicine, Auf’m Hennekamp 50, 40225 Duesseldorf, Germany;

关键词: oxidized alginate;    laminin;    hydrogels;    human induced pluripotent stem cells (hiPSC);    neurospheres;    tissue engineering;   

DOI  :  10.3390/biomedicines9030261

来源: DOAJ

【 摘 要 】

Biodegradable hydrogels that promote stem cell differentiation into neurons in three dimensions (3D) are highly desired in biomedical research to study drug neurotoxicity or to yield cell-containing biomaterials for neuronal tissue repair. Here, we demonstrate that oxidized alginate-gelatin-laminin (ADA-GEL-LAM) hydrogels facilitate neuronal differentiation and growth of embedded human induced pluripotent stem cell (hiPSC) derived neurospheres. ADA-GEL and ADA-GEL-LAM hydrogels exhibiting a stiffness close to ~5 kPa at initial cell culture conditions of 37 °C were prepared. Laminin supplemented ADA-GEL promoted an increase in neuronal differentiation in comparison to pristine ADA-GEL, with enhanced neuron migration from the neurospheres to the bulk 3D hydrogel matrix. The presence of laminin in ADA-GEL led to a more than two-fold increase in the number of neurospheres with migrated neurons. Our findings suggest that laminin addition to oxidized alginate—gelatin hydrogel matrices plays a crucial role to tailor oxidized alginate-gelatin hydrogels suitable for 3D neuronal cell culture applications.

【 授权许可】

Unknown