Keratin 19 maintains E-cadherin localization at the cell surface and stabilizes cell-cell adhesion of MCF7 cells

Cell Adhesion & Migration

Van Lam¹  Thuc Phan¹  Sarah Alsharif¹  Meagan Collins¹  Karina Bursch¹  Rachel Milliken¹  Pooja Sharma¹  Sarah Tiufekchiev¹  Arwa Fallatah¹  Christopher B. Raub¹  Byung Min Chung¹  Priya Dohlman¹  Georges Nehmetallah¹ 

[1] The Catholic University of America;

关键词: intermediate filaments;    keratin;    adherens junction;    cell-cell adhesion;    breast cancer;    metastasis;    cell migration;    cell morphology;    keratin 19;   

DOI  :  10.1080/19336918.2020.1868694

来源: DOAJ

【 摘 要 】

A cytoskeletal protein keratin 19 (K19) is highly expressed in breast cancer but its effects on breast cancer cell mechanics are unclear. In MCF7 cells where K19 expression is ablated, we found that K19 is required to maintain rounded epithelial-like shape and tight cell-cell adhesion. A loss of K19 also lowered cell surface E-cadherin levels. Inhibiting internalization restored cell-cell adhesion of KRT19 knockout cells, suggesting that E-cadherin internalization contributed to defective adhesion. Ultimately, while K19 inhibited cell migration and invasion, it was required for cells to form colonies in suspension. Our results suggest that K19 stabilizes E-cadherin complexes at the cell membrane to maintain cell-cell adhesion which inhibits cell invasiveness but provides growth and survival advantages for circulating tumor cells.

【 授权许可】

Unknown