Comparative Genome Analysis of the Photosynthetic Betaproteobacteria of the Genus Rhodocyclus: Heterogeneity within Strains Assigned to Rhodocyclus tenuis and Description of Rhodocyclus gracilis sp. nov. as a New Species

Microorganisms

Fabiola A. Aviles¹  John A. Kyndt¹  Terrance E. Meyer²  Johannes F. Imhoff³  Sven Künzel⁴  Sven C. Neulinger⁵ 

[1] College of Science and Technology, Bellevue University, Bellevue, NE 68005, USA;Department of Biochemistry, University of Arizona, Tucson, AZ 85721, USA;GEOMAR Helmholtz Centre for Ocean Research Kiel, RD3 Marine Symbioses, Düsternbrooker Weg 20, 24105 Kiel, Germany;Max Planck Institute for Evolutionary Biology, 24306 Plön, Germany;omics2view.consulting GbR, 24118 Kiel, Germany;

关键词: Rhodocyclus;    purpureus;    tenuis;    gracilis;    HiPIP;    cobalamin;   

DOI  :  10.3390/microorganisms10030649

来源: DOAJ

【 摘 要 】

The genome sequences for Rhodocyclus purpureus DSM 168^T and four strains assigned to Rhodocyclus tenuis (DSM 110, DSM 111, DSM 112, and IM 230) have been determined. One of the strains studied (IM 230) has an average nucleotide identity (ANI) of 97% to the recently reported genome of the type strain DSM 109 of Rcy. tenuis and is regarded as virtually identical at the species level. The ANI of 80% for three other strains (DSM 110, DSM 111, DSM 112) to the type strain of Rcy. tenuis points to a differentiation of these at the species level. Rcy. purpureus is equidistant from Rcy. tenuis and the new species, based on both ANI (78–80%) and complete proteome comparisons (70% AAI). Strains DSM 110, DSM 111, and DSM 112 are very closely related to each other based on ANI, whole genome, and proteome comparisons but clearly distinct from the Rcy. tenuis type strain DSM 109. In addition to the whole genome differentiation, these three strains also contain unique genetic differences in cytochrome genes and contain genes for an anaerobic cobalamin synthesis pathway that is lacking from both Rcy. tenuis and Rcy. purpureus. Based on genomic and genetic differences, these three strains should be considered to represent a new species, which is distinctly different from both Rcy. purpureus and Rcy. tenuis, for which the new name Rhodocyclus gracilis sp. nov. is proposed.

【 授权许可】

Unknown