Anti-Inflammatory Activity of Fucoidan Extracts In Vitro

Marine Drugs

Damien N. Stringer¹  Ah Young Park¹  Samuel S. Karpiniec¹  J. Helen Fitton¹  Mathew Suji Eapen²  Sukhwinder Singh Sohal²  Tauseef Ahmad³  Rajaraman Eri³  Vanni Caruso⁴  Muhammad Ishaq⁴  Nuri Guven⁴ 

[1] Marinova Pty Ltd., Cambridge, TAS 7170, Australia;Respiratory Translational Research Group, Department of Laboratory Medicine, School of Health Sciences, College of Health and Medicine, University of Tasmania, Launceston, TAS 7248, Australia;School of Health Sciences, University of Tasmania, Launceston, TAS 7248, Australia;School of Pharmacy and Pharmacology, University of Tasmania, Hobart, TAS 7005, Australia;

关键词: fucoidan;    PBMCs;    THP-1;    TNF-α, IL-1β, and IL-6;    Undaria pinnatifida;    Fucus vesiculosus;   

DOI  :  10.3390/md19120702

来源: DOAJ

【 摘 要 】

Fucoidans are sulfated, complex, fucose-rich polymers found in brown seaweeds. Fucoidans have been shown to have multiple bioactivities, including anti-inflammatory effects, and are known to inhibit inflammatory processes via a number of pathways such as selectin blockade and enzyme inhibition, and have demonstrated inhibition of inflammatory pathologies in vivo. In this current investigation, fucoidan extracts from Undaria pinnatifida, Fucus vesiculosus, Macrocystis pyrifera, Ascophyllum nodosum, and Laminaria japonica were assessed for modulation of pro-inflammatory cytokine production (TNF-α, IL-1β, and IL-6) by human peripheral blood mononuclear cells (PBMCs) and in a human macrophage line (THP-1). Fucoidan extracts exhibited no signs of cytotoxicity in THP-1 cells after incubation of 48 h. Additionally, all fucoidan extracts reduced cytokine production in LPS stimulated PBMCs and human THP-1 cells in a dose-dependent fashion. Notably, the 5–30 kDa subfraction from Macrocystis pyrifera was a highly effective inhibitor at lower concentrations. Fucoidan extracts from all species had significant anti-inflammatory effects, but the lowest molecular weight subfractions had maximal effects at low concentrations. These observations on various fucoidan extracts offer insight into strategies that improve their efficacy against inflammation-related pathology. Further studies should be conducted to elucidate the mechanism of action of these extracts.

【 授权许可】

Unknown