Anti-Atherogenic Properties of Allium ursinum Liophylisate: Impact on Lipoprotein Homeostasis and Cardiac Biomarkers in Hypercholesterolemic Rabbits

International Journal of Molecular Sciences

Attila Kertesz¹  Harjit Pal Bhattoa²  Dezso Csupor³  Judit Hohmann³  David D. Haines⁴  Bela Juhasz⁴  Balazs Varga⁴  Mariann Bombicz⁴  Daniel Priksz⁴  Rudolf Gesztelyi⁴  Peter Balogh⁵  Peter Lengyel⁶ 

[1] Department of Cardiology, Faculty of Medicine, University of Debrecen, Debrecen H-4032, Hungary;Department of Laboratory Medicine, Faculty of Medicine, University of Debrecen, Debrecen H-4032, Hungary;Department of Pharmacognosy, Faculty of Pharmacy, University of Szeged, Szeged H-6720, Hungary;Department of Pharmacology, Faculty of Pharmacy, University of Debrecen, Debrecen H-4032, Hungary;Department of Research Methodology and Statistics, Institute of Sectoral Economics and Methodology, University of Debrecen, Debrecen H-4032, Hungary;Institute of Applied Informatics and Logistics, University of Debrecen, Debrecen H-4032, Hungary;

关键词: Allium species;    atherosclerosis;    lipoprotein;    cardiovascular homeostasis;    echocardiography;   

DOI  :  10.3390/ijms17081284

来源: DOAJ

【 摘 要 】

The present investigation evaluates the capacity of Allium ursinum (wild garlic) leaf lyophilisate (WGLL; alliin content: 0.261%) to mitigate cardiovascular damage in hypercholesterolemic rabbits. New Zealand rabbits were divided into three groups: (i) cholesterol-free rabbit chow (control); (ii) rabbit chow containing 2% cholesterol (hypercholesterolemic, HC); (iii) rabbit chow containing 2% cholesterol + 2% WGLL (hypercholesterolemic treated, HCT); for eight weeks. At the zero- and eight-week time points, echocardiographic measurements were made, along with the determination of basic serum parameters. Following the treatment period, after ischemia-reperfusion injury, hemodynamic parameters were measured using an isolated working heart model. Western blot analyses of heart tissue followed for evaluating protein expression and histochemical study for the atheroma status determination. WGLL treatment mediated increases in fractional shortening; right ventricular function; peak systolic velocity; tricuspidal annular systolic velocity in live animals; along with improved aortic and coronary flow. Western blot analysis revealed WGLL-associated increases in HO-1 protein and decreases in SOD-1 protein production. WGLL-associated decreases were observed in aortic atherosclerotic plaque coverage, plasma ApoB and the activity of LDH and CK (creatine kinase) in plasma. Plasma LDL was also significantly reduced. The results clearly demonstrate that WGLL has complex cardioprotective effects, suggesting future strategies for its use in prevention and therapy for atherosclerotic disorders.

【 授权许可】

Unknown