Fast Fabrication of Solid-State Nanopores for DNA Molecule Analysis

Nanomaterials

Dexian Ma¹  Lijian Zhan¹  Jingjie Sha¹  Yin Zhang¹  Zengdao Gu¹ 

[1] Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing 211189, China;

关键词: solid-state nanopore;    dielectric breakdown;    biosensor;    DNA detection;   

DOI  :  10.3390/nano11092450

来源: DOAJ

【 摘 要 】

Solid-state nanopores have been developed as a prominent tool for single molecule analysis in versatile applications. Although controlled dielectric breakdown (CDB) is the most accessible method for a single nanopore fabrication, it is still necessary to improve the fabrication efficiency and avoid the generation of multiple nanopores. In this work, we treated the SiNx membranes in the air–plasma before the CDB process, which shortened the time-to-pore-formation by orders of magnitude. λ-DNA translocation experiments validated the functionality of the pore and substantiated the presence of only a single pore on the membrane. Our fabricated pore could also be successfully used to detect short single-stranded DNA (ssDNA) fragments. Using to ionic current signals, ssDNA fragments with different lengths could be clearly distinguished. These results will provide a valuable reference for the nanopore fabrication and DNA analysis.

【 授权许可】

Unknown