Interactions of Osteoprogenitor Cells with a Novel Zirconia Implant Surface

Journal of Functional Biomaterials

Dileep Sharma¹  Thomas Munro¹  Catherine M. Miller²  Elsa Antunes³ 

[1] College of Medicine and Dentistry, James Cook University, 14-88 McGregor Road, Smithfield, QLD 4878, Australia;College of Public Health, Medical and Veterinary Sciences, James Cook University, 14-88 McGregor Road, Smithfield, QLD 4878, Australia;College of Science & Engineering, James Cook University, 1 James Cook Drive, Douglas, Townsville QLD 4814, Australia;

关键词: dental implants;    Yttria-tetragonal zirconia;    titanium;    osseointegration;    surface properties;    immunocytochemistry;   

DOI  :  10.3390/jfb11030050

来源: DOAJ

【 摘 要 】

Background: This study compared the in vitro response of a mouse pre-osteoblast cell line on a novel sandblasted zirconia surface with that of titanium. Material and Methods: The MC3T3-E1 subclone 4 osteoblast precursor cell line was cultured on either sandblasted titanium (SBCpTi) or sandblasted zirconia (SBY-TZP). The surface topography was analysed by three-dimensional laser microscopy and scanning electron microscope. The wettability of the discs was also assessed. The cellular response was quantified by assessing the morphology (day 1), proliferation (day 1, 3, 5, 7, 9), viability (day 1, 9), and migration (0, 6, 24 h) assays. Results: The sandblasting surface treatment in both titanium and zirconia increased the surface roughness by rendering a defined surface topography with titanium showing more apparent nano-topography. The wettability of the two surfaces showed no significant difference. The zirconia surface resulted in improved cellular spreading and a significantly increased rate of migration compared to titanium. However, the cellular proliferation and viability noted in our experiments were not significantly different on the zirconia and titanium surfaces. Conclusions: The novel, roughened zirconia surface elicited cellular responses comparable to, or exceeding that, of titanium. Therefore, this novel zirconia surface may be an acceptable substitute for titanium as a dental implant material.

【 授权许可】

Unknown