Propolis Affects Pseudomonas aeruginosa Growth, Biofilm Formation, eDNA Release and Phenazine Production: Potential Involvement of Polyphenols

Microorganisms

Diego Pinetti¹  Stefania Benvenuti²  Federica Pellati²  Elisabetta Blasi³  Bruna Colombari³  Agron Meto⁴  Aida Meto⁵  Lucia Marchetti⁵  Giorgia Boaretto⁶ 

[1] Centro Interdipartimentale Grandi Strumenti, University of Modena and Reggio Emilia, via Campi 213/A, 41125 Modena, Italy;Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, Italy;Department of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Via G. Campi 287, 41125 Modena, Italy;Department of Therapy, Faculty of Dental Medicine, University of Aldent, Rr. e Dibres 235, 1000 Tirana, Albania;School of Doctorate in Clinical and Experimental Medicine, University of Modena and Reggio Emilia, Via G. Campi 287-103, 41125 Modena, Italy;School of Specialization in Microbiology and Virology, University of Modena and Reggio Emilia, Via G. Campi 287, 41125 Modena, Italy;

关键词: p. aeruginosa;    virulence factors;    phenazines;    edna;    mic;    antimicrobial;    antibiofilm;    propolis;    polyphenols;   

DOI  :  10.3390/microorganisms8020243

来源: DOAJ

【 摘 要 】

Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen responsible for a wide range of clinical conditions, from mild infections to life-threatening nosocomial biofilm-associated diseases, which are particularly severe in susceptible individuals. The aim of this in vitro study was to assess the effects of an Albanian propolis on several virulence-related factors of P. aeruginosa, such as growth ability, biofilm formation, extracellular DNA (eDNA) release and phenazine production. To this end, propolis was processed using three different solvents and the extracted polyphenolic compounds were identified by means of high performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC-ESI-MS) analysis. As assessed by a bioluminescence-based assay, among the three propolis extracts, the ethanol (EtOH) extract was the most effective in inhibiting both microbial growth and biofilm formation, followed by propylene glycol (PG) and polyethylene glycol 400 (PEG 400) propolis extracts. Furthermore, Pseudomonas exposure to propolis EtOH extract caused a decrease in eDNA release and phenazine production. Finally, caffeic acid phenethyl ester (CAPE) and quercetin decreased upon propolis EtOH extract exposure to bacteria. Overall, our data add new insights on the anti-microbial properties of a natural compound, such as propolis against P. aeruginosa. The potential implications of these findings will be discussed.

【 授权许可】

Unknown