Heliyon | |
Determination of in vitro antioxidant activity and in vivo antineoplastic effects against Ehrlich ascites carcinoma of methanolic extract of Sphagneticola calendulacea (L.) Pruski | |
Md. Wasim Bari1  Rashida Afroz1  Mst Julia Sultana1  Mohammad Ismail Hossain1  Mohammad Amirul Islam1  Md. Monirul Islam2  Salina Shaheen Parul3  Ariful Islam4  A.M. Swaraz5  Md. Mahbubur Rahman Khan6  | |
[1] Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, 6205, Bangladesh;Department of Biochemistry, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka 431-3192, Japan;Department of Biochemistry, Sylhet MAG Osmani Medical College, Sylhet, Bangladesh;Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka 431-3192, Japan;Department of Genetic Engineering and Biotechnology, Jashore University of Science and Technology, Jashore 7408, Bangladesh;Department of Neurophysiology, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka 431-3192, Japan; | |
关键词: Sphagneticola calendulacea; Antioxidant activity; Antineoplastic activity; Ehrlich ascites carcinoma; Apoptosis; | |
DOI : | |
来源: DOAJ |
【 摘 要 】
Background and aim: Sphagneticola calendulacea (L.) Pruski (S. calendulacea) is a well-known medicinal plant that is widely used in traditional medicine. This study was conducted to evaluate the antioxidant and antineoplastic properties of S. calendulacea leaf. Experimental procedure: Methanol was used as a solvent to prepare leaf extract from the dried leaf of S. calendulacea (MESL). We have evaluated the total phenolic and flavonoid contents of MESL, and using five different assays; we have also evaluated the antioxidant property of MESL. In vivo antineoplastic activity of MESL against Ehrlich ascites carcinoma (EAC) cells in experimental mice was also explored. Results: The phytochemical analysis of MESL exhibited the total phenolic, and total flavonoid contents in the dry extracts were 32.86 mg/gm of gallic acid equivalent and 127.26 mg/gm of catechin equivalent, respectively. Moreover, MESL also showed promising scavenging activity in all assays (IC50 value ≥70.5 μg/mL). In the in vivo antineoplastic assay, MESL showed increased EAC cell death at the doses of 50 mg/kg and 100 mg/kg body weight. MESL administration also induced apoptosis of EAC cells, significantly inhibited EAC cell growth, and increased the life span of EAC cell-bearing mice compared to EAC cell-bearing control mice. Conclusions: All findings of this study suggest potential antioxidant and antineoplastic properties of MESL.
【 授权许可】
Unknown