High-Sensitive TRBC1-Based Flow Cytometric Assessment of T-Cell Clonality in Tαβ-Large Granular Lymphocytic Leukemia

Cancers

Neus Villamor¹  Margarida Lima²  Sheila Mateos³  Carolina Caldas³  Noemí Muñoz-García³  Alberto Orfao³  Susana Barrena³  Julia Almeida³  F. Javier Morán-Plata³  Jacques J. M. van Dongen³ 

[1] Biomedical Research Networking Centre Consortium of Oncology (CIBERONC), Instituto de Salud Carlos III, 28029 Madrid, Spain;Department of Hematology, Laboratory of Cytometry, Hospital de Santo António, Centro Hospitalar do Porto, 4099-001 Porto, Portugal;Translational and Clinical Research Program, Centro de Investigación del Cáncer and IBMCC (CSIC—University of Salamanca), Cytometry Service, NUCLEUS, Department of Medicine, University of Salamanca (USAL) and Institute of Biomedical Research of Salamanca (IBSAL), 37007 Salamanca, Spain;

关键词: TRBC1;    JOVI-1;    TCRVβ repertoire;    large granular lymphocytic leukemia;    LGLL;    large granular lymphocytes;   

DOI  :  10.3390/cancers14020408

来源: DOAJ

【 摘 要 】

Flow cytometric (FCM) analysis of the constant region 1 of the T-cell receptor β chain (TRBC1) expression for assessing Tαβ-cell clonality has been recently validated. However, its utility for the diagnosis of clonality of T-large granular lymphocytic leukemia (T-LGLL) needs to be confirmed, since more mature Tαβ cells (i.e., T-LGL normal-counterpart) show broader TRBC1⁺/TRBC1⁻ ratios vs. total Tαβ cells. We compared the distribution and absolute counts of TRBC1⁺ and TRBC1⁻ Tαβ-LGL in blood containing polyclonal (n = 25) vs. clonal (n = 29) LGL. Overall, polyclonal TRBC1⁺ or TRBC1⁻ Tαβ-LGL ranged between 0.36 and 571 cells/μL (3.2–91% TRBC1⁺ cells), whereas the clonal LGL cases showed between 51 and 11,678 cells/μL (<0.9% or >96% TRBC1⁺ cells). Among the distinct TCRVβ families, the CD28⁻ effector-memory and terminal-effector polyclonal Tαβ cells ranged between 0 and 25 TRBC1⁺ or TRBC1⁻ cells/μL and between 0 and 100% TRBC1⁺ cells, while clonal LGL ranged between 32 and 5515 TRBC1⁺ or TRBC1⁻ cells/μL, representing <1.6% or >98% TRBC1⁺ cells. Our data support the utility of the TRBC1-FCM assay for detecting T-cell clonality in expansions of Tαβ-LGL suspected of T-LGLL based on altered percentages of TRBC1⁺ Tαβ cells. However, in the absence of lymphocytosis or in the case of TαβCD4-LGL expansion, the detection of increased absolute cell counts by the TRBC1-FCM assay for more accurately defined subpopulations of Tαβ-LGL-expressing individual TCRVβ families, allows the detection of T-cell clonality, even in the absence of phenotypic aberrations.

【 授权许可】

Unknown