Use of a Rotating Square Spatial-Frequency Filter to Map the Optical Path Length Variation in Microscopic Biological Samples

Sensors

Ignacio Iglesias¹ 

[1] Departamento de Física, CEIR Campus Mare Nostrum (CMN), Universidad de Murcia, 30100 Murcia, Spain;

关键词: microscopy;    quantitative microscopy;    wavefront sensing;   

DOI  :  10.3390/s22051842

来源: DOAJ

【 摘 要 】

Gradient images can be obtained using a rotating square mask to filter the angular spectra of the wavefront generated by a complex transmittance object. This method can be applied to measure the three-dimensional structure of microscopic biological samples through the relationship of the phase with the optical path length. This work describes the implementation of a system using an inverted optical microscope and shows the experimental results of phase maps generated by boar sperm cells.

【 授权许可】

Unknown