Poly-L-Lysine and Human Plasmatic Fibronectin Films as Proactive Coatings to Improve Implant Biointegration

Frontiers in Bioengineering and Biotechnology

Hassan Rammal¹  Houda Morakchi-Goudjil²  Rémy Agniel²  Anamar Miranda²  Adeline Gand²  Damien Seyer²  Emmanuel Pauthe²  Carla Palomino-Durand²  Mathilde Massonie² 

[1] EFOR Healthcare Paris, Biocompatibility Platform, Levallois-Perret, France;Equipe de Recherche sur les Relations Matrice Extracellulaire Cellules, Institut des Matériaux, CY Cergy-Paris Université, Cergy-Pontoise, France;

关键词: multilayer thin films;    fibronectin;    biomimetic;    bioadhesive;    antimicrobial activity;   

DOI  :  10.3389/fbioe.2021.807697

来源: DOAJ

【 摘 要 】

The success of stable and long-term implant integration implies the promotion, control, and respect of the cell microenvironment at the site of implantation. The key is to enhance the implant–host tissue cross talk by developing interfacial strategies that guarantee an optimal and stable seal of soft tissue onto the implant, while preventing potential early and late infection. Indeed, implant rejection is often jeopardized by lack of stable tissue surrounding the biomaterial combined with infections which reduce the lifespan and increase the failure rate of implants and morbidity and account for high medical costs. Thin films formed by the layer-by-layer (LbL) assembly of oppositely charged polyelectrolytes are particularly versatile and attractive for applications involving cell–material contact. With the combination of the extracellular matrix protein fibronectin (Fn, purified from human plasma) and poly-L-lysine (PLL, exhibiting specific chain lengths), we proposed proactive and biomimetic coatings able to guarantee enhanced cell attachment and exhibiting antimicrobial properties. Fn, able to create a biomimetic interface that could enhance cell attachment and promote extracellular cell matrix remodeling, is incorporated as the anionic polymer during film construction by the LbL technic whereas PLL is used as the cationic polymer for its capacity to confer remarkable antibacterial properties.

【 授权许可】

Unknown