Procedures for cryogenic X-ray ptychographic imaging of biological samples

IUCrJ

U. Wagner¹  F. Cacho-Nerin¹  I. K. Robinson²  F. Zhang²  J. Schwenke²  M. Yusuf²  B. Chen²  A. Bhartiya²  K. Cunnea³ 

[1] Diamond Light Source, Didcot, Oxfordshire, England;London Centre for Nanotechnology, University College London, London, England;Research Complex at Harwell, Rutherford Appleton Laboratory, Oxfordshire, England;

关键词: human chromosomes;    cell nuclei;    chromosome imaging;    X-ray ptychography;    plunge freezing;   

DOI  :  10.1107/S2052252516020029

来源: DOAJ

【 摘 要 】

Biological sample-preparation procedures have been developed for imaging human chromosomes under cryogenic conditions. A new experimental setup, developed for imaging frozen samples using beamline I13 at Diamond Light Source, is described. This manuscript describes the equipment and experimental procedures as well as the authors' first ptychographic reconstructions using X-rays.

【 授权许可】

Unknown